Figure 3. Torsional stress affects nucleosome dynamics and Pol II kinetics. Genes were grouped based on change in torsional stress as measured by change in TMP crosslinking before and after Topo I or II inhibition. The highest (High TD) and lowest (Low TD) 20% of genes are shown. For these two groups, the change in nucleosome turnover (A), the change in Pol II pausing (B), and the change in Pol II elongation as measured by nascent RNA production (C) are plotted for regions surrounding the TSS.