Figure 1. Our pipeline for RNA-seq analysis in almond under control and frost stress conditions.

Two almond tissues (anther and ovary) were subjected to untreated and −2°C conditions. Following the RNA exteraction, cDNA library was constructed and sequencing was performed using an IIlumina platform. De novo transcript assembly was conducted after quality control and trimming the reads and then contigs were annotated. Transcript aboundance and differentially expressed genes were analyzed using RSEM and EBseq, respectively. Finally, gene ontology were determined for each set of genes in the DE list.