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. 2014 Aug 14;9(8):e104541. doi: 10.1371/journal.pone.0104541

Table 2. Statistics of de novo transcriptome assembly and annotation.

Sample Num. ofcontigs Min. length(bp) Max. length(bp) N50 Annotation byBLASTX (%) Reads mapped topeach genome (%) Reads mapped back tocontigs (%)
HSA 44477 200 11888 1484 94.54 80.73 79.42
HSO 41283 200 6888 1558 93.16 79.75 87.23
HCA 38920 200 7145 1204 91.63 81.26 82.39
HCO 48562 200 8256 1444 93.49 79.22 85.69

De novo assembley was performed using Trinity package following the standard manual. Annotation was achieved using BLASTX program. Mapping statistics of reads to the transcriptome assemblies and peach genome are also provided, which was obtained using Bowtie v2 package.

Abbreviations: HSA, stressed anther of H genotype; HCA, control anther of H genotype; HSO, stressed ovary of H genotype; HCO, control ovary of H genotype.