Table 2. Statistics of de novo transcriptome assembly and annotation.
Sample | Num. ofcontigs | Min. length(bp) | Max. length(bp) | N50 | Annotation byBLASTX (%) | Reads mapped topeach genome (%) | Reads mapped back tocontigs (%) |
HSA | 44477 | 200 | 11888 | 1484 | 94.54 | 80.73 | 79.42 |
HSO | 41283 | 200 | 6888 | 1558 | 93.16 | 79.75 | 87.23 |
HCA | 38920 | 200 | 7145 | 1204 | 91.63 | 81.26 | 82.39 |
HCO | 48562 | 200 | 8256 | 1444 | 93.49 | 79.22 | 85.69 |
De novo assembley was performed using Trinity package following the standard manual. Annotation was achieved using BLASTX program. Mapping statistics of reads to the transcriptome assemblies and peach genome are also provided, which was obtained using Bowtie v2 package.
Abbreviations: HSA, stressed anther of H genotype; HCA, control anther of H genotype; HSO, stressed ovary of H genotype; HCO, control ovary of H genotype.