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. 2014 Aug 14;9(8):e104583. doi: 10.1371/journal.pone.0104583

Figure 4. Slowly migrating forms of SOD1 in BN-gels do not react with C4F6 or SEDI antibody.

Figure 4

Immunoblots of BN-gels containing the S1 fraction from lysates of cells expressing WT, A4V, and G93A SOD1 (see Methods) were probed with the hSOD1, SEDI, and C4F6 antibodies. Membranes were incubated in acetic acid to fix the proteins to the membrane and then incubated with antibodies as described in Methods. All membranes were developed in parallel with equivalent exposure times, both short and long exposures are provided to allow visualization of weak reactivity of the G93A variant with the C4F6 antibody. No reactivity to the A4V variant was detected with the C4F6 or SEDI antibody. The images shown are representative of at least 3 independent replications.