Table 1. Primers used for qPCR to detect the heat tolerance-related SNP all-53308-760 T/C.

Index	Sequences*	Amplification efficiency**
all-53308 forward	ATGGATCTCAGAAGTGAACGT	0.998
all-53308 reverse	ATGATTCTAGCCTGTACCATTAC
all-53308-T forward	GGATCTCAGAAGTGAACGTCTcAT	1.02
all-53308-C forward	GGATCTCAGAAGTGAACGTCTtAC	0.958
18S rRNA forward	CTGACCATAAACGATGCCGACT	0.938
18S rRNA reverse	AACTTTGGTTTCCCGTAAGCTG

*The bases bold are an allele-specific base, and the bases in lowercase are a deliberate mismatch.

**The amplification efficiency of the allele-specific forward primer was determined in combination with all-53308 reverse primer.