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. 2014 Aug 14;9(8):e104883. doi: 10.1371/journal.pone.0104883

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© 2014 Luchting et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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PBMCs extracellular stained with PerCP labeled anti-human CD4-antibody, PE labeled anti CD25-antibody, Brilliant Violet (BV570) labeled anti CD127-antibody and intracellular stained with Alexa Fluor (AF488) labeled anti-human FoxP3-antibody. Lymphocyte population was gated from PBMCs according to forward scatter (FSC) characteristics and side scatter (SSC) characteristics (left). Gated lymphocytes were then separated in CD4⁺CD25^high cells/T cells (middle) and CD4⁺CD25^highCD127^lowFOXP3⁺ cells/CD4⁺T cells (right, named Treg). Upper row represents the result of a healthy control with less CD4⁺CD25^high T cells (3.28%) and less CD4⁺CD25^highCD127^lowFoxP3⁺ T cells (1.94%) compared to a patient with CLBP (lower row, 5.74% and 3.11%).