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. 2014 Aug 14;9(8):e104883. doi: 10.1371/journal.pone.0104883

Figure 6. Flow cytometric quantification of Tregs and TH17 cells.

Figure 6

Results show significantly higher percentage of anti-inflammatory Tregs in patients with CLBP in both staining protocols (CD4+CD25high cells: 4.45±0.88% in CLBP patients vs. 3.49±0.53% in healthy controls, p<0.001; Fig. 6A), (CD4+CD25highCD127lowFoxP3+ cells: 2.89±1.07% in CLBP patients vs. 1.93±0.66% in healthy controls, p = 0.001; Fig. 6B). Number of TH17 cells as percentage of T cells in peripheral blood show significantly lower percentage of pro-inflammatory TH17 cells in patients with CLBP (TH17 cells: 0.46±0.24% in CLBP patients vs. 1.14±0.73% in healthy controls, p<0.001; Fig. 6C).