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. 2014 Aug 14;9(8):e104571. doi: 10.1371/journal.pone.0104571

Figure 4. Localization of HetC-GFP.

Figure 4

(A) Scheme of the genomic hetC region of strains CSM1 (expressing a HetC-GFP-mut2 fusion protein) and CSL33 (expressing a HetC-p-GFP-mut2 fusion protein). The pCSV3 vector portion integrated in the hetC locus is represented as a thin line. (B) Scheme of the putative domains of the HetC protein. (C) Confocal microscopy of filaments of strains CSM1 and CSL33 grown in bubbled, ammonium-supplemented medium and incubated for 24 h in medium containing no combined nitrogen. Cyanobacterial autofluorescence (red) is shown in the right-hand images, and merged autofluorescence and GFP fluorescence (green) in the left-hand images. Heterocysts (indicated with white arrows) are identified by their greatly diminished autofluorescence.