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. 2014 Aug 14;9(8):e104571. doi: 10.1371/journal.pone.0104571

Figure 5. Localization of HetP-GFP.

Figure 5

(A) Scheme of the hetP genomic region of strains CSL67 (HetP-sf-GFP fusion protein) and CSL107 (HetP-GFP-mut2 fusion protein). The pCSV3 vector portion integrated in the hetP locus is represented as a thin line. (B) Scheme of the HetP protein showing a predicted transmembrane segment (TMS). (C) Confocal microscopy of filaments of strain CSL67 grown in BG110 solid medium (upper part) or deconvoluted fluorescence microscopy image of filaments of strain CSL67 grown in bubbled ammonium-supplemented medium and incubated for 40 h in medium containing no combined nitrogen (lower part). (D) Fluorescence microscopy of filaments of strain CSL107 grown in BG110 solid medium. Merged images of autofluorescence and GFP fluorescence are shown at the left side, and of bright field and GFP fluorescence at the right side. Heterocysts and some proheterocysts are indicated with white arrows.