Table 2. Plasmids used in this work.

Name	Description	Strain generated	Reference
pCSAL39	sf-gfp with N-terminal 4Gly linker-encoding sequence and BsaI site in a modified pMBL-T (Ap resistance substituted by C.K1 cassette)	-	A. López-Lozano and A. Herrero (unpublished)
pCSAM135	Contains a translational fusion between the 5′-terminal region of sepJ and the gfp gene		Flores et al. 2007
pCSL18	PCR product obtained with primers alr2817-34, alr2817-35, alr2817-36 and alr2817-37 (overlapping PCR), cloned in pMBL-T	-	This work
pCSL19	C.S3 inserted in EcoRV between the two DNA fragments of pCSL18	-	This work
pCSL20	pCSL19 digested with BamHI and XhoI, and cloned in pRL278	CSL3	This work
pCSL22	PCR product obtained with primers alr5358-1, alr5358-2, alr5358-3 and alr5358-4 (overlapping PCR), cloned in pMBL-T	-	This work
pCSL23	pCSL22 digested with BamHI and XhoI and cloned in pRL278	CSL7	This work
pCSL24	PCR product obtained with primers alr2817-38, alr2817-42, alr2817-43 and alr2817-41 (overlapping PCR), cloned in pMBL-T	-	This work
pCSL25	pCSL24 digested with SpeI and XbaI and cloned in pCSRO (XbaI)	CSL16	This work
pCSL68	pCSV3-derived plasmid containing the mut2-gfp gene	-	This work
pCSL69	PCR product obtained with primers alr2818-8 and alr2818-9, cloned in pCSAL39 digested with BsaI and HindIII	-	This work
pCSL70	pCSL69 digested with KpnI and cloned in pCSV3 digested with the same enzyme	CSL67	This work
pCSL111	PCR product obtained with primers asr2818-13, asr2819-1, asr2819-2 and alr2820-1 (overlapping PCR), cloned in pCSRO digested with SacI	CSL97	This work
pCSL123	PCR product obtained with primers alr2818-19, GFP-13, GFP-14 and GFP-11 (overlapping PCR), cloned in pCSV3 digested with KpnI	CSL107	This work
pCSM6	hetC PCR product obtained with primers alr2817-13 and alr2817-14 fused to mut2-gfp through an EcoRV site, cloned in PstI-digested pCSV3	CSM1	This work
pCSRO	Plasmid derived from pRL278, with KmR gene substituted by C.S3	-	Merino-Puerto et al. (2010)
pCSV3	Plasmid derived from pRL500, with ApR gene substituted by C.S3	-	Olmedo-Verd et al. (2006)
pMBL-T	Commercial vector for cloning purposes	-	Dominion MBL
pRL278	Vector used for the positive selection of double recombinants in Anabaena	-	Black et al. (1993)