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. 2014 Aug 14;9(8):e104838. doi: 10.1371/journal.pone.0104838

Figure 2. Promoter reporter studies for reported NR5A1 mutations.

Figure 2

. Human embryonic kidney HEK293 cells were transiently transfected with wild-type (WT) or mutant SF-1 and promoter luciferase reporter constructs of the genes for steroidogenic enzymes HSD3B2 (A), CYP11A1 (B), CYP17A1 (C). Luciferase activity was measured with the Promega Dual Luciferase assay system. Results are expressed as percentage of WT SF-1 activity. Independent experiments were performed in duplicate at least 3 times. Error bars represent the mean and SEM. *p<0.05; **p<0.01.