ChIP-seq of chromatin precipitated with antibodies against GFP-tagged CenH3 (purple), H3K4me2 (green), H3K9me3 (red) and H3K27me3 (orange) suggests that H3K27me3 acts as the major gene silencing modification on Fusarium B chromosomes (Sung-Hwan Yun, Lena Studt, Bettina Tudzynski, Lanelle Connolly, Kristina Smith and Michael Freitag, preliminary data). Note the predicted acrocentric centromeres in both species, either determind by Cen H3 ChIP (purple in F. fujikuroi) or predicted by large region of H3K9me3 (red, F. fujikuroi).