Skip to main content
. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Biochem Biophys Res Commun. 2014 Mar 22;450(3):1212–1217. doi: 10.1016/j.bbrc.2014.03.083

Figure 1. Effect of fertilization on PYK2 activation.

Figure 1

Groups of 20–30 zona-free oocytes were fertilized in vitro then washed free of unbound sperm and solubilized for western blot analysis as described in “Materials and Methods”. Blots were probed with anti PYK2 PY579 (panel A, D), anti-FAK PY861 (panel B, E), or anti-active SRC (panel C, F). Bound antibody was detected by chemiluminescence and films were scanned to quantify band intensity which was corrected for loading errors by GAPDH content and expressed relative to the band intensity in unfertilized oocytes (Panels D–F). (n) indicates the number of oocyte groups that were analyzed. (*) indicates the value was significantly different from that in unfertilized oocytes (P<0.05).