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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Hypertension. 2014 Jun 16;64(3):573–582. doi: 10.1161/HYPERTENSIONAHA.114.03663

Figure 5. Effect of the sex of the T cell on Th17 polarizing T cell responses to Ang II.

Figure 5

Lymph node mononuclear cells were harvested from male and female WT mice (N=5/group) and stimulated with anti-CD3 and anti-CD28 under Th0 (no added cytokines) (A&C) or under Th17-polarization conditions (treatment with IL-6, TGFβ, anti-IL-4 and anti-IFNγ) (B&D) in the presence of vehicle (water) or Ang II (100 nM). Cytokine production by lymph node cells was measured using cytokine ELISA kits (A&B) and intracellular cytokine analyses (C&D). Cytokine levels are expressed as means ± SEM in triplicate cultures (A&B) or as the frequencies of cytokine producing cells in the live CD4+ gate in pooled cells from triplicate cultures (C&D); Cytokine gates were set using fluorescence minus one controls. Cytokine data were analyzed by two-way ANOVA to identify sex by treatment interactions. This was followed by one-way ANOVA and Tukey post-hoc test to determine individual differences between groups. *P≤0.05 vs. vehicle, same sex.