Figure 4. C12orf4 is implicated in the early events of the degranulation pathway.

a) Western blot analysis of the FcεRI-mediated phosphorylation of major proteins implicated in mast cell activation. 5H4 expressing RBL-2H3 cells are compared to cells expressing an irrelevant antibody fragment. Cells are either non-activated or activated with IgE/DNP for 3 and 10 minutes as indicated. b) Schematic view of mast cell signaling pathways, kindly provided by Dr. Marc Daëron.