Table 1. Selected F. hepatica proteins containing signal peptide (SP) but no transmembrane domain (TM).
Epitope | Synthesised peptide sequence | Position | GenBank | Description | Proteinlength (aa) |
B1 | KGAGSSQDACIKFIQYEVDG | 63–82 | AAB02579.1 | Amoebapore homologue | 102 |
B2 | KGAGSSQDATIKFIQYEVDG | 63–82 | AAB02579.1 | Amoebapore homologue | 102 |
B3 | FASFDVPSKQPTIDIDLCDI | 14–33 | AAF88069.1 | Amoebapore-like protein | 101 |
B4 | FASFDVPSKQPTIDIDLTDI | 14–33 | AAF88069.1 | Amoebapore-like protein | 101 |
B5 | ISEIRDQSSTSSTWAVSSAS | 102–121 | ABU62951.1 | Cathepsin B | 337 |
B6 | GVENGVKYWLIANSWNEGWG | 293–312 | ABU62951.1 | Cathepsin B | 337 |
B7 | QTCSPLRVNHAVLAVGYGTQ | 260–279 | AAB41670.2 | Secreted cathepsin L1 | 326 |
260–279 | AAA29136.1 | Cathepsin | 326 | ||
264–283 | AAP49831.1 | Cathepsin L | 326 | ||
260–279 | Q24940.1 | Cathepsin L-like proteinase | 326 | ||
B8 | QTTSPLRVNHAVLAVGYGTQ | 260–279 | AAB41670.2 | Secreted cathepsin L1 | 326 |
260–279 | AAA29136.1 | Cathepsin | 326 | ||
264–283 | AAP49831.1 | Cathepsin L | 326 | ||
260–279 | Q24940.1 | Cathepsin L-like proteinase | 326 | ||
B9 | YTEPRSVTPEERSVFQPMIL | 27–46 | AAV68752.1 | Cystatin | 116 |
B10 | FVPLYSSKSATSVGTPTRVS | 95–114 | AAV68752.1 | Cystatin | 116 |
B11 | VTTNGPPNGKHNDKHTYVEC | 350–369 | CAC85636 | Legumain-like | 419 |
B12 | VTTNGPPNGKHNDKHTYVET | 350–369 | CAC85636 | Legumain-like | 419 |
T13 | TVNLVKRLLQNSVVE | 37–51 | AAB02579.1 | Amoebapore homologue | 102 |
T14 | DYIIDHVDQHNATEI | 80–94 | AAF88069.1 | Amoebapore-like protein | 101 |
T15 | DRNTQRQTVRYSVSE | 69–83 | ABU62925.1 | Cathepsin B | 337 |
T16 | FYMFEDFLVYKSGIY | 260–274 | ABU62925.1 | Cathepsin B | 337 |
T17 | KYLTEMSRASDILSH | 83–97 | AAB41670.2 | Secreted cathepsin L1 | 326 |
83–97 | ABQ95351.1 | Secreted cathepsin L2 | 326 | ||
83–97 | AAA29136.1 | Cathepsin | 326 | ||
83–97 | AAP49831.1 | Cathepsin L | 326 | ||
83–97 | AAR99518.1 | Cathepsin L protein | 326 | ||
83–97 | Q24940.1 | Cathepsin L-like proteinase | 326 | ||
T18 | ISFSEQQLVDTSGPW | 153–167 | AAB41670.2 | Secreted cathepsin L1 | 326 |
153–167 | AAA29136.1 | Cathepsin | 326 | ||
153–167 | AAP49831.1 | Cathepsin L | 326 | ||
153–167 | BAA23743.1 | Cathepsin L | 325 | ||
153–167 | AAR99518.1 | Cathepsin L protein | 326 | ||
153–167 | AAT76664.1 | Cathepsin L1 proteinase | 326 | ||
153–167 | Q24940.1 | Cathepsin L-like proteinase | 326 | ||
T19 | ENAYEYLKHNGLETE | 178–192 | AAC47721.1 | Secreted cathepsin L2 | 326 |
178–192 | ABN50361.2 | Cathepsin L | 326 | ||
178–192 | CAA80446.1 | Cathepsin L-like protease | 326 | ||
53–67 | CAA80445.1 | Cathepsin L-like protease | 166 | ||
T20 | LDPYFNLVSPEVYNY | 29–43 | BAE44988.1 | Cytochrome oxidase subunit I | 145 |
29–43 | BAE45005.1 | Cytochrome oxidase subunit I | 145 | ||
T21 | DLNLPRLNALSAWLL | 76–90 | BAE44988.1 | Cytochrome oxidase subunit I | 145 |
76–90 | BAE45005.1 | Cytochrome oxidase subunit I | 145 | ||
T22 | FAGHGKAYLHGSFDK | 56–70 | AAA29144.1 | Vitelline protein B2 | 272 |
T23 | YEKYEDDYARETPYD | 254–268 | AAA29144.1 | Vitelline protein B2 | 272 |
254–268 | AAA29143.1 | Vitelline protein B1 | 272 | ||
T24 | 101–115 | AAA31753.2 | NADH dehydrogenase subunit 3 | 118 | |
101–115 | Q34522.1 | NADH dehydrogenase subunit 3 | 118 | ||
101–115 | AAG13152.2 | NADH dehydrogenase subunit 3 | 118 |
GenBank accession number and amino acid sequences from each B- and T-cell chemically synthesised epitope are also indicated.