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. 2014 Aug 14;9(8):e105323. doi: 10.1371/journal.pone.0105323

Table 1. Selected F. hepatica proteins containing signal peptide (SP) but no transmembrane domain (TM).

Epitope Synthesised peptide sequence Position GenBank Description Proteinlength (aa)
B1 KGAGSSQDACIKFIQYEVDG 63–82 AAB02579.1 Amoebapore homologue 102
B2 KGAGSSQDATIKFIQYEVDG 63–82 AAB02579.1 Amoebapore homologue 102
B3 FASFDVPSKQPTIDIDLCDI 14–33 AAF88069.1 Amoebapore-like protein 101
B4 FASFDVPSKQPTIDIDLTDI 14–33 AAF88069.1 Amoebapore-like protein 101
B5 ISEIRDQSSTSSTWAVSSAS 102–121 ABU62951.1 Cathepsin B 337
B6 GVENGVKYWLIANSWNEGWG 293–312 ABU62951.1 Cathepsin B 337
B7 QTCSPLRVNHAVLAVGYGTQ 260–279 AAB41670.2 Secreted cathepsin L1 326
260–279 AAA29136.1 Cathepsin 326
264–283 AAP49831.1 Cathepsin L 326
260–279 Q24940.1 Cathepsin L-like proteinase 326
B8 QTTSPLRVNHAVLAVGYGTQ 260–279 AAB41670.2 Secreted cathepsin L1 326
260–279 AAA29136.1 Cathepsin 326
264–283 AAP49831.1 Cathepsin L 326
260–279 Q24940.1 Cathepsin L-like proteinase 326
B9 YTEPRSVTPEERSVFQPMIL 27–46 AAV68752.1 Cystatin 116
B10 FVPLYSSKSATSVGTPTRVS 95–114 AAV68752.1 Cystatin 116
B11 VTTNGPPNGKHNDKHTYVEC 350–369 CAC85636 Legumain-like 419
B12 VTTNGPPNGKHNDKHTYVET 350–369 CAC85636 Legumain-like 419
T13 TVNLVKRLLQNSVVE 37–51 AAB02579.1 Amoebapore homologue 102
T14 DYIIDHVDQHNATEI 80–94 AAF88069.1 Amoebapore-like protein 101
T15 DRNTQRQTVRYSVSE 69–83 ABU62925.1 Cathepsin B 337
T16 FYMFEDFLVYKSGIY 260–274 ABU62925.1 Cathepsin B 337
T17 KYLTEMSRASDILSH 83–97 AAB41670.2 Secreted cathepsin L1 326
83–97 ABQ95351.1 Secreted cathepsin L2 326
83–97 AAA29136.1 Cathepsin 326
83–97 AAP49831.1 Cathepsin L 326
83–97 AAR99518.1 Cathepsin L protein 326
83–97 Q24940.1 Cathepsin L-like proteinase 326
T18 ISFSEQQLVDTSGPW 153–167 AAB41670.2 Secreted cathepsin L1 326
153–167 AAA29136.1 Cathepsin 326
153–167 AAP49831.1 Cathepsin L 326
153–167 BAA23743.1 Cathepsin L 325
153–167 AAR99518.1 Cathepsin L protein 326
153–167 AAT76664.1 Cathepsin L1 proteinase 326
153–167 Q24940.1 Cathepsin L-like proteinase 326
T19 ENAYEYLKHNGLETE 178–192 AAC47721.1 Secreted cathepsin L2 326
178–192 ABN50361.2 Cathepsin L 326
178–192 CAA80446.1 Cathepsin L-like protease 326
53–67 CAA80445.1 Cathepsin L-like protease 166
T20 LDPYFNLVSPEVYNY 29–43 BAE44988.1 Cytochrome oxidase subunit I 145
29–43 BAE45005.1 Cytochrome oxidase subunit I 145
T21 DLNLPRLNALSAWLL 76–90 BAE44988.1 Cytochrome oxidase subunit I 145
76–90 BAE45005.1 Cytochrome oxidase subunit I 145
T22 FAGHGKAYLHGSFDK 56–70 AAA29144.1 Vitelline protein B2 272
T23 YEKYEDDYARETPYD 254–268 AAA29144.1 Vitelline protein B2 272
254–268 AAA29143.1 Vitelline protein B1 272
T24 101–115 AAA31753.2 NADH dehydrogenase subunit 3 118
101–115 Q34522.1 NADH dehydrogenase subunit 3 118
101–115 AAG13152.2 NADH dehydrogenase subunit 3 118

GenBank accession number and amino acid sequences from each B- and T-cell chemically synthesised epitope are also indicated.