Table 4. Gene expression analysis of epithelial CCH cells after co-culture with miR-132 overexpressing fibroblasts.

15 most upregulated genes	Fold change(log2)	15 mostdownregulatedgenes	Fold change (log2)
TFEC	5.90	NEK9	−5.53
MARCH9	3.95	SLMAP	−4.98
EPB41L3	3.92	DOCK5	−4.96
DNAH11	3.60	CUL4B	−4.80
NEK11	3.45	AP3B1	−4.79
IFFO1	3.41	C1orf9	−4.78
GLUL	3.40	PLEKHA5	−4.72
TAF1C	3.40	ZZEF1	−4.65
ACSS2	3.32	DHRS9	−4.64
NCRNA00114	3.30	BZW2	−4.61
WNK3	3,30	ULK2	−4,49
TRMT2B	3,22	SLTM	−4,44
PIWIL3	3,16	ADAMTS16	−4,40
MAP2	3,16	POT1	−4,31
RASGRP3	3,13	TBCK	−4,30
Significantly upregulated pathways	P †	Ratio‡	Genes
Cellular Effects of Sildenafil (Viagra)	0.001	5/151	SLC4A5, MYH2, MYH8, ITPR1, MYH1
Calcium Signaling	0.012	4/207	MYH2, MYH8, ITPR1, MYH1
Actin Cytoskeleton Signaling	0.023	4/238	MYH2, MYH8, NCKAP1L, MYH1
Hepatic Fibrosis/Hepatic Stellate CellActivation	0.035	3/147	MYH2, MYH8, MYH1
Assembly of RNA Polymerase I Complex	0.045	1/13	TAF1C
Tight Junction Signaling	0.046	3/164	MYH2, MYH8, MYH1
Significantly downregulated pathways	P †	Ratio‡	Genes
Glycerophospholipid Metabolism	0.003	7/179	GPAM, PLCB2, BCHE, DGKB, DGKG, LPIN2, LYPLA1
Glycerolipid Metabolism	0.005	6/148	GPAM, DHRS9, LIPF, DGKB, DGKG, LPIN2
Phospholipid Degradation	0.008	5/93	PLCB2, DGKB, DGKG, LPIN2, LYPLA1
Cellular Effects of Sildenafil (Viagra)	0.022	6/151	MYH4, PLCB2, ADCY2, CACNA1E, PDE4C, MYH7
G-Protein Coupled Receptor Signaling	0.024	15/528	PLCB2, ADCY2, CCKAR, PDE4C, OPN1LW, SOS2, PDE6C, GPR107, HTR6, RGS12, GPR64, CAMK2D, DRD1, RXFP1, BAI1
cAMP-mediated signaling	0.025	8/218	ADCY2, CAMK2D, PDE4C, DRD1, PDE6C, CNGB1, HTR6, RGS12
Purine Metabolism	0.033	9/392	ADCY2, POLR3B, PDE4C, KIF1B, NUDT9, PDE6C, MYH7, REV3L, RALBP1

^† P was calculated by the Ingenuity System.

^‡Ratio: altered genes/total #genes in pathway.

Results are presented as log2 fold change.