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. 2014 Aug 14;8(8):e3079. doi: 10.1371/journal.pntd.0003079

Figure 2. NO tolerizes B. pseudomallei to the antimicrobial activity of imipenem.

Figure 2

(A) All bacteria in groups I-III were treated with 25 µg/ml imipenem for 2.5 h. B. pseudomallei in group I were treated with 100 µM spermine NONOate (sNO) 1 h after exposure to imipenem; sNO was removed from group II after 1 h of treatment; sNO was maintained in group III during the 2.5 h of challenge. The survival of control bacteria (C) treated with 25 µg/ml of imipenem is shown for comparison. Panel B shows the fraction of B. pseudomallei that survived 25 µg/ml imipenem at the indicated times. Selected specimens (dark symbols) were treated with 100 µM sNO during the first hour of exposure to imipenem. The data are the mean ± SEM from 5 observations collected on 2 separate days. p<0.001 compared to groups I–III.