Figure 6. Modulation of antimicrobial activity of imipenem by NO produced by IFNγ-activated J774 cells.

Killing of B. pseudomallei (BPS) and Salmonella (STM) in IFNγ-activated J774 cells treated with increasing concentrations of imipenem (A and D, respectively). Where indicated, 500 µM of the iNOS inhibitor aminoguanidine (AG) was added to the cells. NO₂ ⁻ produced by the macrophages was estimated by the Griess reaction (B and E). Panel C shows the killing of log phase B. pseudomallei treated for 2.5 h in LBG broth with 500 µM spermine NONOate (sNO) and/or 0.25 µg/ml imipenem (IM).