Figure 6. Epidermal APCs trigger IFN-γ production by CD8⁺ T_EFF cells.

(A,B) Analysis of IFN-γ⁺ in vitro activated gBT-I (A,B) and gDT-II (B) T_EFF cells cultured (as in Figure 5 ) with increasing numbers of CD45.2⁻ and CD45.2⁺ APCs sorted from epidermal sheets (pooled from 20 mice) 4 days after HSV-1 skin infection. Data representative (A) or pooled from 2–3 experiments (B). (C,D) Analysis of IFN-γ⁺ in vitro activated gBT-I cells cultured with sorted APCs from epidermal sheets (pooled from 20 mice) 4 days after infection. APC numbers were 5×10⁴ for keratinocytes (KC), CD11c⁺ and residual CD45.2⁺CD11c⁻Vγ3⁻ (CD45.2⁺) cells; 3×10⁴ for DETCs. Data representative (C) or pooled from 2 experiments (D). **, P<0.01 by student t-test individually comparing specific APC subsets to control conditions (No APC).