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. 2014 Aug 14;10(8):e1004248. doi: 10.1371/journal.ppat.1004248

Figure 2. ENV TMD inhibits secretion of cytokines and chemokines induced by TLR2.

Figure 2

(A–C) RAW cells were incubated with/without the indicated peptides for 2 hours, then washed twice and activated with 500 ng hours, then washed twice and activated with 500 ng ng/ml of purified LTA. Media was collected after 5 hours (TNFα) or 24 hours (MCP-1 and IL-6). ELISA was performed using standard kits. (n = 3, ± represent SD) (D) THP-1 human macrophages were incubated using similar conditions as described in A, and TNFα levels were measured (* - p<0.05).