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. 2014 Aug 14;10(8):e1004248. doi: 10.1371/journal.ppat.1004248

Figure 3. HIV1-ENV intact protein inhibits LTA mediated TNFα secretion.

Figure 3

RAW cells were transfected either with vector plasmid (YFP) or gp160 HIV-1 ENV expressing plasmid or gp41 HIV-1 ENV expressing plasmid as indicated in materials and methods section, and after 48 hours of transfection cells were activated with 500 ng hours of transfection cells were activated with 500 ng ng/ml of purified LTA, and TNFα secretion levels were measured by ELISA and compared to non-treated RAW (naïve) cells. Results are from 3 typical experiments and each is a triplicate, *, p<0.05.