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. 2014 Aug 14;10(8):e1004326. doi: 10.1371/journal.ppat.1004326

Figure 5. ECTV-Δ005 still inhibits p65 nuclear accumulation.

Figure 5

(A) HeLa cells were mock-infected or infected with ECTV or ECTV-Δ005 at a MOI of 5. At 12 hours post infection, cells were stimulated with 10 ng hours post infection, cells were stimulated with 10 ng ng/ml TNFα for 20 minutes, fixed and stained with anti minutes, fixed and stained with anti-p65 and DAPI. Cells were visualized by immunofluorescence. (B) HeLa cells or (C) MEF cells were mock-infected or infected with ECTV or ECTV-Δ005 at a MOI of 5. At 12 hours post infection, cells were stimulated with 10 ng hours post infection, cells were stimulated with 10 ng ng/ml TNFα for 20 minutes, and nuclear and cytoplasmic extracts were collected. Protein samples were separated by SDS minutes, and nuclear and cytoplasmic extracts were collected. Protein samples were separated by SDS-PAGE and western blotted with anti-p65, anti-β-tubulin, anti-PARP and anti-I5L.