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. Author manuscript; available in PMC: 2014 Aug 14.
Published in final edited form as: Aging Cell. 2010 Oct;9(5):799–809. doi: 10.1111/j.1474-9726.2010.00619.x

Figure 8. bcl-x Gene Promoter Activity by PG Treatment.

Figure 8

Primary cultured cardiomyocytes were transfected with luciferase constructs under the control of −905 bp human bcl-x promoter (A) or −3.2 kb mouse bcl-x promoter (B) using Fugene6 liposomes. At 48 hrs after transfection, cells were placed in 0.5% FBS for 24 hrs before treatment with 1 μM corticosterone (CT, A), 10 μM PG (A) or PG at indicated doses (B) for 24 hrs before harvesting for measurement of luciferases. A superscript letter indicates significant difference (P<0.05) from means labeled with a different letter as determined by ANOVA analysis.