TABLE 2. Pubertal Landmark Age (PND) (Mean ± SE).

Males	n	Testes descent	Preputial separation
Naïve control	12	22.79 ± 0.19	44.88 ± 0.80
Vehicle control	12	23.13 ± 0.21	45.21 ± 0.75
2.5 BPA	11	23.00 ± 0.32	44.45 ± 0.49
25.0 BPA	12	22.88 ± 0.22	44.25 ± 0.52
5.0 EE2	11	27.27 ± 0.78*	52.50 ± 1.35*
10.0 EE2	11–12a	32.88 ± 1.27*	70.45 ± 4.24*
Females	n	Vaginal opening
Naïve control	12	35.96 ± 0.77
Vehicle control	12	35.95 ± 0.88
2.5 BPA	11	34.18 ± 0.66
25.0 BPA	12	36.55 ± 0.69
5.0 EE2	7b	45.43 ± 5.98*
10.0 EE2	8c	42.94 ± 3.39*

^aBoth male siblings from one 10.0 EE₂ dam never experienced preputial separation. One male from a separate 10.0 EE₂ dam also never experienced preputial separation, although its same-sex sibling did at PND 78. Thus, n = 11 for preputial separation.

^bBoth female siblings from four 5.0 EE₂ dams never experienced vaginal opening. One female each from three separate 5.0 EE₂ dams also never experienced vaginal opening, although their same-sex sibling did. Thus, n = 7 for this treatment group.

^cBoth female siblings from four 10.0 EE₂ dams never experienced vaginal opening. One female from a separate 10.0 EE₂ dam also never experienced vaginal opening, although its same-sex sibling did. Thus, n = 8 for this treatment group.

*Significantly later than same-sex vehicle controls.