Fig. 6.

Male (A) and female (B) liver vtg mRNA expression measured by qRT/RT-PCR (mean ± SEM). Fold induction is expressed relative to controls within each time point after normalization to 18S rRNA by the 2^−ΔΔCq method. After 4 weeks of exposure, female fish from 10 μg/l BaP had a significant induction of vtg compared with 1 μg/l BaP but not compared with controls (ANOVA on 2^−ΔCq, p < 0.05; n = 6 tanks with 3 fish/tank at 4 weeks; n = 5 tanks with 2–3 fish/tank at 2 weeks; bars with the same letters are not significantly different).