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Differentiation and experimental scheme for functional characterization of human embryonic stem cell (ES cell)-derived hepatocytes: (A) protocol for hepatocyte differentiation from ES cells, drug induction, metabolism, and toxicity testing; (B) ES cell-derived hepatocytes in adherent culture. Suspension culture (for 72 h) of: (C) ES cell-derived hepatocytes, (D) primary hepatocytes, (E) HepG2 cells, and (F) 293 cells.
