TabLE 1. Genes Differentially Expressed Between Adherent and Suspension Cultured ES-Hepatocytes.

Gene	H1 ES cells	HepG2	Primary hepatocytesa	Adherent ES hepatocytes	Suspension ES hepatocytes	log2 fold changeb
SERPINA1	0.00	3439.17	6166.76	0.00	439.31	8.78
KRT8	389.03	311.59	294.54	0.00	5048.34	12.30
G6PC	0.40	66.31	130.73	0.02	55.05	5.78
HSD17B13	1.55	1.18	219.10	0.81	36.38	4.37
CYP1A1	0.00	6.22	4.25	2.75	37.74	3.37
FN1	8.22	350.13	332.62	1755.14	21879.27	3.64
SGK1	5.87	30.73	130.25	0.95	112.21	5.86
SGK2	1.29	7.53	13.56	2.56	21.87	2.68
C3	5.21	421.10	1898.51	92.27	677.13	2.86
PRODH2	0.00	83.72	76.54	1.90	11.05	2.05
SLC30A1	1.31	11.10	21.74	0.24	42.54	5.13
SLC25A3	513.95	417.24	126.74	0.00	26.14	4.76
SLC25A4	18.25	11.65	12.09	0.23	27.28	4.52
SLC16A1	35.12	82.12	31.13	1.13	10.87	2.48
SLC25A18	0.63	0.23	48.63	1.07	6.11	1.78
SLC22A3	0.00	8.48	12.54	0.72	62.09	5.20
SLC6A13	0.00	0.37	12.77	0.77	4.53	1.64
SLC25A18	0.63	0.23	48.63	1.07	6.11	1.78
UGT1A1	0.00	0.56	114.66	0.00	3.25	2.09
UGT1A6	0.00	0.08	264.35	1.57	5.18	1.27
UGT1A9	0.00	0.00	56.83	0.00	0.00	0.00
UGT2B15	0.77	250.68	309.52	6.30	10.64	0.67
ALB	0.00	46062.04	86272.40	247.52	63.72	−1.94
ABCB1	0.11	19.20	10.57	9.62	0.03	−3.37
AFP	0.68	9412.51	21.27	11413.50	3786.49	−1.59

Note. Gene expression values are in transcripts per million (TPM).

^a Average of primary human hepatocyte batches 1–5 are reported.

^b A pseudocount of 1 is added to the numerator and denominator before calculating log₂((tpm x)/(tpm y)).