Fig. 3.

Sn facilitates marginal metallophilic macrophage trapping of GBS to limit pathogen dissemination. WT and Sn-deficient mice were intravenously injected with 5-(and-6)-carboxyfluorescein labeled GBS, and kidney, lung, and spleen were collected 1 h post-infection. Sections were stained with mAb anti-mouse Sn, F4/80, and B220. Representative images of spleen sections are shown in a and b. Scale bar is 100 μm for all panels except 20 μm for the right panel of a. Comparison of bacterial counts (expressed in CFU) recovered from the kidney (c), lung (d), and spleen (e) of WT mice and Sn-deficient mice 1 h post-infection. Difference between two groups was calculated by unpaired t test. Representative data (n = 3–4 mice each group) were shown from two independent experiments