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. 2014 Aug 15;4:6016. doi: 10.1038/srep06016

Figure 4. Identification and stoichiometric quantitation of PKBα phosphorylated peptides by LC-MS analysis.

Figure 4

Extracted-ion chromatograms of four tryptic peptide ions extracted from LC-MS chromatograms after BAP treatment (+BAP) or no BAP treatment (No BAP). The sequences (possible phosphorylated residues, underlined) and m/z values are indicated. The peak height was normalized against the unphosphorylated ion in +BAP or the phosphorylated ion in No BAP. The dotted line indicates the stoichiometry of the phosphorylation level determined from the BAP treatment. (a) Peptide mass and mass windows: [TFCGTPEYLAPEVLEDNDYGR]3+; 816.03 Da ± 10 ppm, and [pTFCGpTPEYLAPEVLEDNDYGR]3+; 869.33 Da ± 10 ppm. (b) Peptide mass and mass windows: [RPHFPQFSYSASSTA]2+; 841.90 Da ± 10 ppm, and [RPHFPQFpSYSASSTA]2+; 881.88 Da ± 10 ppm.