Figure 2.

Genome-wide distribution of histone modifications. Distribution of (A) H3K27me3 and (B) H3K27ac with respect to gene features in A. gambiae midguts. The enrichment or depletion is shown relative to chromatin input. The regions in the map comprise gene bodies flanked by a segment of 200 bp at the 5′ end of TSSs and TTSs. Average profiles across gene regions ±200 bp for each histone modification are shown on top. (C) Heatmap of RNA-seq data showing the level gene expression, as read count, profiled along the region. In all heatmaps (A–C) genes were organized into 3 clusters based on their level of H3K27me3. For H3K27ac, cluster genes are ordered by descent but independent to H3K27me3. The color bars indicate the range of intensities based on ChIP enrichment, from red to blues for higher to low enrichment values. Boxplots showing the mean enrichment of H3K27ac and H3K27me3 by cluster (D), and mean level of gene expression by gene cluster (E). Significant pairwise comparisons are indicated by asterisks (see text).