Fig. 2.

a–c Agarose gels showing amplified tissue DNA in patients #2–4; rearranged DNA was not detected in plasma of these patients (not shown). d Agarose gel showing amplified rearranged DNA in plasma after 50 cycles of PCR (patient #1); the DNA sequence of the band was sequenced and was found to be identical with the DNA of the lymphoma tissue. e Amplified rearranged tissue DNA of patient #1. Of note, the designed PCR product is 153 bp; the size is different from the rearranged DNA fragment in plasma (d) as different primers were used for tissue and plasma (positions of tissue and plasma primers that were used are illustrated in Supplement Fig. 1). Normal tissue DNA was used as negative control