Figure 2.

Akt1 is required for initiation and progression of lung tumors induced by mutant K-ras. Mice harbored a latent activatible mutant K-ras (K-ras^LA2/+). (a) Lung tumor multiplicity at 16 weeks. Each point represents one mouse; lines are at means. (b) Lung tumor volume increases with time in +/+ but not Akt1^−/− mice. Bars represent mean tumor volume±s.e.m. (c) Representative 16 week-old K-ras^LA2/+ whole mounts of lungs stained with hematoxylin and eosin. Normal lung appears pink and tumors appear dark purple. Notice decreased number and size of lung tumors in the Akt1^−/−/K-ras^LA2/+ lungs. Overall size of lungs (and mice) are decreased in Akt1^−/− mice, which has been described previously.