Figure 1. Ubiquitination Modulates Treg Cell Function.

(A) Sorted human CD4⁺CD25^highCD127^low Treg cells were pretreated with 10 μM DUBi for 1 hr, washed, and cocultured with CFSE-labeled PBMCs in anti-CD3-coated wells for 4 days. CFSE dilution of CD4⁺ cells was analyzed by flow cytometry.

(B) CD4⁺CD45RB^high cells were injected into immunodeficient mice. Sorted GFP⁺ Treg cells from Foxp3-GFP promoter mice were pre-incubated with 10 μM DUBi for 1 hr and injected 3 weeks later. Mice were sacrificed 3 weeks after Treg cell administration. Sections of the colon were analyzed and scored (five mice per group).

(C) Representative hematoxylin- and eosin-stained tissue slides of the colon.

(D) Analysis of CD4⁺GFP⁺ cell numbers in the spleen.

(E) Percentage of Foxp3⁺ CD4⁺ T cells in the spleen. Data shown are representative of at least three independent experiments, *p<0.05.

Data are represented as mean + SEM. See also Figure S1.