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. 2014 Aug 6;5:4619. doi: 10.1038/ncomms5619

Figure 2. Matrix rigidity mediated H3K9 methylation in control cells but not in TRCs.

Figure 2

(a) H3K9 methylation is lower in 90-Pa fibrin gels than on rigid plastic. Control B16-F1 cells were transfected with the H3K9 FRET methylation biosensor and then plated either on gelatin-coated rigid plastic (2D plastic) or in the 90-Pa soft 3D fibrin gel (3D Fibrin). H3K9 methylation was measured at the indicated times after cell plating. Mean±s.e.m.; n>75 cells for each value (*P<0.001). (b) H3K9 methylation in control B16-F1 cells (Control) but not in TRCs increases on stiff PA substrates coated with collagen I (Col-1) but not fibrinogen (Fib). PA, polyacrylamide. H3K9 methylation was measured 15 h after cell plating. Mean±s.e.m., n>40 cells for each value (*P<0.005).Representative FRET images of a cell nucleus for control cells (c) and TRCs (d) on PA gels coated with collagen I (top panel)) or fibrinogen (bottom panel). Scale bars, 5 μm.