Fig. 2. p38 MAPK is required for leptin expression induced by HER2. HER2-MCF10A cells were pretreated with 50 μM SB203580 for 24 hr. Expression of leptin, phosphorylated p38 (pp38) MAPK, and total p38 MAPK were detected in cell lysates by immunoblot analysis. Band intensities were quantified and plotted. The results are means + S.E. triplicates. *Statistically different at P ＜ 0.05.
