Fig. 3. Invasive phenotype induced by HER2 requires leptin expression. (A) HER2-MCF10A cells were transfected with control siRNA or siRNA targeting leptin. Leptin knock-down was confirmed by immunoblot analysis (left) and RT-PCR analysis (right). Band intensities were quantified and plotted. The results are means + S.E. of triplicates. *Statistically different at P ＜ 0.05. (B) Cells were subjected to the in vitro invasion assay. The number of invaded cells per field was counted (×400) in 13 arbitrary visual fields. The results represent means + S.E. of triplicates. *Statistically different from control at P ＜ 0.05.
