Fig. 4. Leptin plays a crucial role in the expressions of MMP-2 and MMP-9 in HER2-MCF10A cells. HER2-MCF10A cells were transfected with control siRNA or siRNA targeting leptin. Conditioned media were collected, and gelatinolytic activities of secreted MMP-2 and MMP-9 were determined by gelatin zymogram assay. Band intensities were quantified and plotted. The results are means + S.E. of triplicates. *Statistically different at P ＜ 0.05.
