Fig. 2. Expression pattern of KAI1 mRNA during trophoblast differentiation. BeWo cells were cultured and differentiated using forskolin (50 μM). After 24 or 72 h, cells were examined to determine mRNA expressions. KAI1 and β-Actin mRNA levels were determined by reverse-transcription PCR (A) and those of KAI1, hCG, and GAPDH were determined by real-time RT-PCR (B). Empty and black bars indicate control and forskolin, respectively. Two independent experiments were performed. Each sample was triplicated and represented as means ± SD. *P ＜ 0.05 and **P ＜ 0.01.