Fig. 2. The effect of ROS scavengers on salsolinol-mediated cytochrome c aggregation and carbonyl compound formation. 20 μM cytochrome c was incubated with 0.5 mM salsolinol in 10 mM phosphate buffer (pH 7.4) at 37℃ for 24 h in the presence of ROS scavengers. (A) The pattern of protein bands was analyzed via SDS-PAGE. Lane 1, cytochrome c control; lane 2, oxidized cytochrome c (without ROS scavenger); lane 3, 100 mM azide; lane 4, 100 mM formate; lane 5, 20 mM glutathione (GSH); lane 6, 20 mM N-acetyl cysteine (NAC). (B) The formation of carbonyl compounds was determined by spectrophotometry.
