Fig. 4. Modification of amino acid residues in cytochrome c by salsolinol. Cytochrome c was incubated in 10 mM potassium phosphate buffer (pH 7.4) at 37℃ with the following conditions: 20 μM cytochrome c alone (dark bar); 20 μM cytochrome c plus 0.5 mM salsolinol (gray bar). After incubation for 24 h, the amino acid composition of acid and alkaline hydrolysates was determined, as described in Materials and Methods.
