Fig. 1. Schematic diagram and purification of the recombinant albumin fusion proteins. (A) Fusion plasmids were genetically engineered: GLP-1 human serum albumin fusion (GLP-1/HSA), AGLP-1/HSA, which has an additional Ala at the N-terminal location of GLP-1, and AGLP-1-L/HSA in which a peptide linker is inserted. The plasmids for the fusion protein were transiently transfected into CHO-K1. (B) The fusion proteins were purified by Ni-affinity chromatography and analyzed using 10% (v/v) SDS-PAGE stained with Coomassie Brilliant Blue-G (CBB-G) under reducing conditions. M, Broad range protein marker; Lane 1, GLP-1/HSA; Lane 2, AGLP-1/HSA; Lane 3, AGLP-1-L/HSA.