Fig. 2. Identification of the hEC-SOD gene for transgenic chicken. (A) PCR transgene analysis. M, 100-bp DNA ladder; Pc, positive control using the OVP-hEC-SOD/pLenti6/TR plasmid; lanes 1-7, genomic DNA samples isolated from the semen of the hatched roosters (G0); b, before EcoRI digestion; a, after EcoIRI digestion. One rooster (lane 6) was positive for the transgene in the germline. The red arrowheads indicate the EcoRI-digested amplified DNA fragments (a) or the undigested amplified DNA fragment (b). (B) Southern blot analysis of transgenic chickens. Genomic DNA samples extracted from the whole blood of wild-type and transgenic chickens (G1 and G2) were digested with EcoRV, electrophoresed, blotted, and hybridized with the probe for the hEC-SOD gene. M, DIG-labeled DNA marker; lanes 1 and 2, wild-type chickens; lane 3, the G435; lane 4, the H148; lanes 5-7, the G435’s offspring; lanes 8-10, the H148’s offspring. (C) Nucleotide sequences flanking the hEC-SOD provirus. Small letters, the chicken cAMP-dependent protein kinase type I-beta regulatory subunit gene; bold capital letters, the provirus.