Fig. 2. Effect of floridoside on the viability and activation of BV-2 cells. (A) Effect of floridoside on viability of BV-2 cells. Cells were serum starved for 1 h and treated with LPS (100 ng/ml) followed by the treatment with floridoside (1, 10 and 50 μM) 24 h. Cell viability was assessed by MTT assay. (B) Time dependent NO inhibitory effect of floridoside. Cells were cultured in serum-free media and treated with LPS (100 ng/ml) for 1 h and then incubated with floridoside (1, 10 and 50 μM) for 50 h. Conditioned media was collected at different times (18, 24 and 50 h) and NO production levels were compared. (C) Inhibitory effects of different concentrations (1, 10 and 50 μM) of floridoside on LPS-induced NO production in BV-2 cells. Conditioned medium was collected after 24 h and NO concentration was measured using the Griess reaction. Standard nitrite curve was used to quantify the NO production level. Data are presented as means ± standard deviation (SD) of three independent experiments (n = 3 total). ^a-eMeans with different letters are significantly different (P ＜ 0.05) according toTukey b multiple range test.