Fig. 1. Cytokine production was induced through TLR4 and TLR2 on mouse mast cells. The mBMMCs from Tlr2-/- mice (A), Tlr4-/- mice (B), and their wild type (Tlr2+/+ or Tlr4+/+) were stimulated for 6 hr with Pam3CSK4 (2.5 μg/ml) or LPS (200 ng/ml, smooth type from E. coli serotype O4). As a negative control, buffer alone was used instead of PAMP stimuli. Mouse IL-6 levels in the supernatant were determined by ELISA. Data are represented as means ± S.D. from triplicate experiments. Results are representative of three independent experiments that yield similar results. Asterisk indicates the statistical significance versus controls: *P ＜ 0.01.
