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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Dev Neurobiol. 2013 Nov 20;74(3):333–350. doi: 10.1002/dneu.22141

Figure 5.

Figure 5

Exogenous expression of the mCherry+3′UTR [38bp] transgene in the frontal cortex leads to increased cortical ROS levels. (A) Expression of the mCherry+3′UTR [38bp] in brain sections containing the frontal cortex from transgenic mice and non-transgenic littermates was visualized by fluorescence microscopy using Alexa488 (Green) after immunostaining with dsRED antibody. The ROS levels in the frontal cortex were visualized by oxidized DHE (red) in sections counterstained with 4′,6-diamidino-2-phenylindole (blue). (B) Confocal micrographs showing neurons in the frontal cortex that were double-labeled with mCherry (green) and DHE (red). Arrowheads show neurons that show mCherry expression (dsRED:Green), as well as elevated ROS levels (DHE: Red). The merged confocal images show a single optical section. (C) Relative ROS levels in the frontal cortex neurons were quantified from fluorescence images and compared to the ROS levels of non-transgenic littermates. Values represent the mean ± SEM. Students t test, *, p < 0.01.