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. Author manuscript; available in PMC: 2014 Aug 15.
Published in final edited form as: Immunobiology. 2013 Aug 21;219(1):53–63. doi: 10.1016/j.imbio.2013.08.003

Fig. 8.

Fig. 8

Schematic representation of the alternative splicing of the Cr2 gene seen in WT and Cr2KO animal lines. The first intron sequence of 4818 bp in the WT Cr2 gene is present in both the Cr2KO and Cr1KO lines (denoted by shaded black box). The genomic span between the signal sequence (SS) exon and that encoding SCR1 is 5392 bp and SS to that encoding SCR7/8 is 12,972 bp in the WT gene. The star in the Cr2KO and Cr1KO alleles denotes the insertion site of the Neo gene sequence which, during germline propagation, resolves to a single Lox site. The Cr1KO allele does not demonstrate any alternative splice suggesting the alternative splicing target sequence(s) is within coding sequences for SCRs 1–6, not the promoter or first intron of the Cr2 gene. The position of the hypothetical location within the exons for SCRs 1–6 is indicated by the black arc and asterisk.