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. 2014 Jul 22;15:15. doi: 10.1186/1471-2199-15-15

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Copyright © 2014 Sørensen et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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The different DNA fragments (=bricks) in the USER-Brick vector system. The ends of the Bricks are colour coded based on which overhangs that are compatible for fusion. Top panel: The core USER-Brick includes backbones, selection markers, promoters and fluorescent marker fragments. Centre panel: The placement of the different types of PCR amplicons in relation to the gene of interest. Bottom panel: Sequences of the 5′ overhang found on the primers for amplifying the different USER-Bricks in the two panels above.