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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Genes Immun. 2014 Feb 6;15(3):137–144. doi: 10.1038/gene.2014.3

Figure 6. Mutation of the most proximal STAT5 binding site in the CD154 promoter diminishes CD154 transcription.

Figure 6

Primary human CD4 T cells were transfected with a WT, or BS#1 STAT5-mutant version, CD154 promoter-driven firefly luciferase reporter plasmid. After a brief rest period, the CD4 T cells were activated with P+I overnight, and cell lysates were analyzed for firefly luciferase activity controlled for transfusion efficiency with a co-transfected control Renilla luciferase reporter plasmid. Results are representative of 4 similar experiments and SEM bars are depicted.