. Author manuscript; available in PMC: 2014 Nov 1.

Published in final edited form as: J Microbiol Methods. 2013 Aug 19;95(2):149–155. doi: 10.1016/j.mimet.2013.08.007

Table 2.

Sequence and coverage rate of forward primers.

Primer Name	Primer Sequence (5′ → 3′)	Primer Length (bp)	% RDP predicted coverage^b
Primer Name	Primer Sequence (5′ → 3′)	Primer Length (bp)	M=0	M=1	M=2
799F	AACMGGATTAGATACCCKG	19	81.8	88.1	95.9
799F-mod1	AACMGGATTAGATACCCKGG	20	81.6	88.1	95.8
799F-mod2	AACMGGATTAGATACCCKGGT	21	81.4	88.0	95.7
799F-mod3	CMGGATTAGATACCCKGG	18	82.1	88.2	95.9
799F-mod4	GGATTAGATACCCKGG	16	82.4	95.5	99.3
799F-mod5	ATTAGATACCCKGG	14	82.7	95.7	99.5
799F-mod6	CMGGATTAGATACCCKGGT	19	81.9	88.1	95.9
799F-mod7	GGATTAGATACCCKGGT	17	82.2	95.4	99.3
799F-mod8	ATTAGATACCCKGGT	15	82.5	95.6	99.4
799F-mod9	TAGATACCCKGGT	13	85.8	95.8	99.6
799F-thio	AACMGGATTAGATACCCKG	19	81.8	88.1	95.9
799F-mod2thio	AACMGGATTAGATACCCKGGT	21	81.4	88.0	95.7
799F-mod4thio	GGATTAGATACCCKGG	16	82.4	95.5	99.3
799F-mod7thio	GGATTAGATACCCKGGT	17	82.2	95.4	99.3

Underlined bases are bonded with phosphorothioate bonds. The number of mismatches (M), zero, one, or two, allowed between the primer and target region in a RDP search.